Background: Becoming a parent for the first time has a life changing impact for both the mother as well as father. First time fatherhood is a time of great change, stress and transition.During past decades fatherhood has been changed in many ways as father and children interact more with each other and now a days, fathers play an active role in caring for their child. Aim: The main aim of the study was to enhance the knowledge of first time fathers and to increase their participation in neonatal care. Objectives: To assess the pre-existing knowledge. To assess the effectiveness of planned teaching programme and to assess the association of pre test and post knowledge score with demographic variables. Methods and materials: Quantitative approach was adopted using the pre-experimental design. The sample size was 60 first time fathers were selected by purposive sampling method. Data was collected by using self structured knowledge questionnaire. After conducting pre-test, PTP was administered to the participants. Post-test was conducted after one week. Statistical analysis of the data was done by calculating mean, median, mean percentage, mean difference, standard deviation, t test and chi square test. Result: The present study reveal that, in pre-test knowledge score maximum 40(66.7%) had moderate knowledge, whereas 3 (5%) had adequate knowledge and 17(28.3%) had inadequate knowledge. In post-test, knowledge score, maximum 52(86.7%) had adequate, 8(13.3%) had moderate knowledge and no participant, i.e. 0(0%) had inadequate knowledge. Conclusion: The mean post-test knowledge was higher than the mean pre-test knowledge score. Hence, it can be concluded that the planned teaching programme was effective in improving the knowledge of the first time fathers.