Background: Toxoplasma gondii (T. gondii) is a parasitic protozoan that can infect warm-blooded animals, including humans. Cats are the definitive host of T. gondii, meaning that they are the only animals in which the parasite can sexually reproduce. Aim: The study aimed at molecular detection of the SAG4 gene of the Toxoplasma in cancer patients by real time PCR Materials and methods: The study collected 240 samples from cancer patients and recorded information about each patient in a questionnaire from the period 1/11/2022 to 1/4/2023. The samples were kept at -20°C until they were examined using PCR. The DNA extraction kit used guanidine thiocyanate to break down cells and purify nucleic acids for amplification using RT-PCR. The PCR reaction was specific to the SAG-4 gene and monitored using fluorinated dyes and oligonucleotide probes. Positive samples showed curves during the amplification process, while negative samples showed a straight horizontal line. The study used an internal control and detected the presence of T. gondii DNA in the fluorinated HEX channel. Results: The results of the preliminary study showed that 26.25% (63) out of the total patient group of 240 examined had IgG antibodies to toxoplasmosis gondii, while these antibodies were not detected in the control group. The current study found that the mean of CCL2 protein in cancer patients was 69.44 pg/ml), and in the healthy group was 5.394 pg/ml), with a significant difference (P ≤ 0.0001),. The results of the study with the mean level of IL-12 in cancer patients were 29.42, ±20.76 and in the healthy group. 3.353, ±2.585 and there is a significant difference between them (P≤0.0001)