A review of the various aspects of enzyme immobilization is discussed. Being natural catalysts that accelerate specific biochemical reactions, enzymes, in a restricted or confined state has attracted research and industry interest. The advantages of enzyme immobilization are more stability, allows the development of a multi-enzyme reaction system, reduces effluent disposal problems, while the limitations of immobilized enzymes can include its additional bearing on cost, it also invariably affects the stability and activity of enzymes. Immobilized enzymes can be applied in the food, pharmaceutical, medical and manufacturing industry. This review is a highlight of current trends in enzyme immobilization support and applications.