Aim: This study was designed to determine if there is a correlation between celiac disease in children with autism. Materials and Methods: 40 (11 female and 29 male) autistic children aging 4 to 9 years (median age 6.5 years) and 40 healthy age- and sex- matched (11 female and 29 male) were participated in this prospective study, all the patient & control were tested for IgG and IgA anti-tissue transglutaminase antibody and for IgG and IgA antigliadin antibody, none of the patients and controls had symptoms (or positive family history) suggestive of specific gastrointestinal diseases. Results: IgA anti-tissue transglutaminase antibody was found in 3 autistic child (7%) and 2 control (5%) p=0.64, none of the patient and control were positive for other tests (IgG anti-tissue transglutaminase antibody, IgG and IgA antigliadin). Conclusions: The present study demonstrated that gluten sensitivity is not a major cause in those autistic children examined.