Background: The COVID-19 pandemic caused by SARS-CoV-2 has significantly impacted global health systems, prompting widespread diagnostic testing efforts to curb transmission and guide clinical management. Methods: This study analyses laboratory data from RT-PCR testing conducted at the SARS Virus Research and Diagnostic Laboratory (SVRDL) in Government Medical College, Nagpur. A total of 106,178 samples were processed using various RT-PCR kits, including COVIDsure, with results categorized by cycle threshold (CT) values for RdRP, N, and E genes. Results: Among the samples tested, 13,578 were positive for COVID-19. Demographic analysis revealed higher infection rates among males (53.12%) and older age groups, notably 51-60 years. Asymptomatic cases constituted a significant majority (72.11%) of positives, highlighting their potential role in community transmission. Fever emerged as the predominant symptom among symptomatic patients. Conclusion: The findings underscore the importance of targeted testing strategies, especially among high-risk groups such as older adults, and effective public health interventions to mitigate COVID-19 transmission. Understanding the demographic and clinical characteristics of COVID-19 patients is crucial for shaping future pandemic responses and resource allocation.